Protocols
Biohacking Protocols
Quick-reference protocols with links to full guides. Learn the science first, then follow the protocol.
Infrared Sauna Protocol
Strong EvidenceHuberman and Rhonda Patrick's most-cited longevity intervention. 140°F+ for 20+ minutes.
Cold Exposure Protocol
Moderate EvidenceHuberman's minimum effective dose in uncomfortably cold water.
Sleep Optimization Protocol
Strong EvidenceTemperature, light, and tracking. The foundation of every biohack.
Red Light Therapy Protocol
Moderate EvidenceMorning photobiomodulation at 660nm + 850nm.
HRV Recovery Protocol
Moderate EvidenceTrain based on recovery, not motivation.
CGM Metabolic Protocol
Strong EvidencePersonalized nutrition through real-time glucose data.
NAD+ Longevity Protocol
Emerging EvidenceNR or NMN with TMG methyl donor support.
Creatine Protocol
Strong Evidence5g monohydrate for muscle, brain, and healthy aging.
Magnesium Sleep Protocol
Moderate Evidence200–400mg glycinate 30–60 min before bed.
Blueprint Core Stack
Moderate EvidenceBryan Johnson's evidence-based essentials, not the full 100-pill stack.
Zone 2 Cardio Protocol
Strong EvidencePeter Attia's mitochondrial foundation: conversational pace cardio.
Breathwork Protocol
Moderate EvidenceBox breathing, Wim Hof, and physiological sigh for nervous system control.
Foundation Supplement Stack
Strong EvidenceCreatine, magnesium, vitamin D+K2, omega-3. The tier-1 stack.
Contrast Therapy Protocol
Moderate EvidenceIntermittent Fasting Protocol
Moderate EvidenceDave Asprey's foundational metabolic biohack: 16:8 with fat-fueled mornings.
Bulletproof Coffee Protocol
Moderate EvidenceCoffee + C8 MCT + grass-fed butter for ketone-fueled focus.
Vagus Nerve Toning Protocol
Moderate EvidenceCold face immersion, slow breathing, and humming for nervous system resilience.
Neurofeedback Protocol
Moderate EvidenceEEG brain training for focus, calm, and meditation depth.
Huberman Sleep Stack
Emerging Evidence50mg apigenin + magnesium + theanine for sleep onset support.
Lion's Mane Nootropic Protocol
Moderate Evidence500–1000mg dual-extract lion's mane for cognitive support.
Ashwagandha Stress Protocol
Moderate Evidence600mg KSM-66 for cortisol reduction and stress resilience.
Blood Panel Baseline Protocol
Strong EvidenceComprehensive biomarker testing with ApoB, insulin, and thyroid.
BPC-157 Recovery Protocol
Emerging Evidence250–500mcg subcutaneous for tissue and gut healing.
TB-500 Recovery Protocol
Emerging Evidence2–2.5mg subcutaneous for tissue migration and joint recovery.
Glycine Sleep Protocol
Moderate Evidence3g glycine powder 30–60 min before bed for core temperature drop.
Circadian Rhythm Protocol
Strong EvidenceMorning sunlight within 60 min of waking, dim lights after sunset.
Grounding Protocol
Emerging EvidenceBarefoot outdoor contact or indoor grounding mat during sleep/work.
Urolithin A Longevity Protocol
Moderate Evidence500mg urolithin A for mitophagy and mitochondrial health.
Collagen Peptides Protocol
Moderate Evidence10–15g hydrolyzed collagen in morning coffee or smoothie.
Blue Light Blocking Protocol
Moderate EvidenceAmber lenses plus dimmed overhead lights for melatonin protection.
Epitalon Longevity Cycle
Emerging Evidence5–10mg subcutaneous for pineal and telomerase support.
Huberman Sleep Stack (Full)
Emerging EvidenceApigenin 50mg + magnesium 200–400mg + theanine 100–200mg.
D3 + K2 Protocol
Moderate Evidence2000–5000 IU D3 + 100–200mcg K2 with fat-containing meal.
Mouth Tape Nasal Breathing
Emerging EvidencePorous vertical tape strip for nasal breathing and snoring reduction.
Berberine Metabolic Protocol
Moderate Evidence500mg berberine per meal for glucose control and AMPK activation.
Rhodiola Morning Protocol
Moderate Evidence200–400mg standardized extract before breakfast for calm energy.
NAC Glutathione Protocol
Moderate Evidence600–1200mg NAC on empty stomach for antioxidant and liver support.
CoQ10 Mitochondrial Protocol
Moderate Evidence100–400mg ubiquinol with fatty meal, especially if on statins.
Tongkat Ali Testosterone Protocol
Moderate Evidence200–400mg standardized extract with baseline and follow-up bloodwork.
LMNT Electrolyte Protocol
Strong Evidence1000mg sodium packet during fasting, sauna, or heavy training.
Oura vs WHOOP Decision Guide
Strong EvidenceCompare sleep tracking, strain coaching, and subscription costs.
Zinc Immune Protocol
Moderate Evidence15–30mg zinc picolinate with food for immune and hormonal support.
Probiotic Gut Protocol
Moderate Evidence10–50B CFU of clinically studied strains with prebiotic fiber.
Cryotherapy Protocol
Moderate EvidenceWhole-body cryo or ice bath for recovery and norepinephrine boost.
Spermidine Autophagy Protocol
Emerging Evidence1–6mg spermidine from wheat germ extract for cellular cleanup.
Shilajit Vitality Protocol
Emerging Evidence250–500mg purified shilajit resin for energy and testosterone support.
Peptides Introduction Protocol
Emerging EvidenceUnderstand peptide classes, sourcing, and safety before starting.
HBOT Recovery Protocol
Moderate EvidenceHyperbaric oxygen at 1.5–2.0 ATA for tissue repair and recovery.
Omega-3 Protocol
Strong Evidence2–3g combined EPA+DHA for cardiovascular and brain health.
Vitamin D Protocol
Strong Evidence2000–5000 IU D3 with K2, test blood levels twice yearly.
Gut Microbiome Protocol
Moderate EvidenceDiverse fiber, fermented foods, and targeted testing.
Testosterone Optimization Protocol
Moderate EvidenceSleep, lifting, zinc, tongkat ali, and baseline blood panels.
Glutathione Support Protocol
Moderate EvidenceNAC, glycine, and sulfur-rich foods for master antioxidant production.
Environmental Health Protocol
Moderate EvidenceAir purification, water filtration, and toxin reduction at home.
Nootropics Starter Protocol
Emerging EvidenceBegin with lion's mane, L-theanine, and caffeine before advanced stacks.
AG1 Daily Greens Protocol
Moderate EvidenceOne scoop greens powder in water each morning for micronutrient coverage.
Sleep Cooling Protocol
Strong Evidence65–68°F bedroom, warm bath 90 min pre-bed, active cooling if needed.
Fisetin Senolytic Pulse
Emerging Evidence1000mg fisetin/day for 2 days to clear senescent cells.
Methylene Blue Micro-Dose
Emerging Evidence0.5–4mg pharma-grade methylene blue — never with SSRIs.
Home Water Filtration
Strong EvidenceTest tap water, install RO or gravity filter for PFAS and lead removal.
GHK-Cu Peptide Protocol
Moderate Evidence1–2mg subQ or topical 1–2% serum for skin and tissue repair.
Stem Cell Therapy Overview
Emerging EvidenceUnderstand stem cell types, clinics, and realistic outcome expectations.